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Updated: May 8, 2026

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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Fluorescence turn-on detection of a protein using cytochrome c as a quencher
Dongli Liao1, Jian Chen, Wenying Li
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
Abstract:
A new approach for the sensitive and selective sensing of a protein has been developed. Cytochrome c could quench the fluorescence of a fluorophore labeled aptamer efficiently. The specific binding between the aptamer and the target protein resulted in a turn-on emission signal, which could be used for protein quantification.
