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Updated: May 8, 2026

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
Published on: June 2, 2022
Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear
Kittappa Shathish1, Chandrasekharan Guruvayoorappan
1Karunya University, Coimbatore, Tamil Nadu, India.
Abstract:
Metastasis is an extremely complex process that is a major problem in the management of cancer. In the present study, we had evaluated the antimetastatic activity of DECALEPIS HAMILTONI: using B16F-10 melanoma-induced experimental lung metastasis in a C57BL/6 mice model.
D Hamiltoni:
treatment significantly ( : < .01) inhibited lung tumor nodule formation and reduced the lung collagen hydroxyproline, hexosamine, and uronic acid levels. Similarly serum sialic acid and γ-glutamyl transpeptidase levels were also significantly inhibited after
D Hamiltoni:
treatment. The levels of proinflammatory cytokines such as tumor necrosis factor α, interleukin (IL)-1β, IL-6, granulocyte monocyte colony-stimulating factor, and IL-2 in the serum of these animals were significantly altered after
D Hamiltoni:
treatment. The serum NO level was also found to be significantly decreased after
D Hamiltoni:
treatment. This decreased NO level after
D Hamiltoni:
treatment was also accompanied by decreased inducible NO synthase and cyclooxygenase-2 expression. The study reveals that
D Hamiltoni:
treatment could alter proinflammatory cytokine production and could inhibit the activation and nuclear translocation of p65 and p50 subunits of nuclear factor κB in B16F-10 cells.
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