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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Updated: May 8, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

targetHub: a programmable interface for miRNA-gene interactions.

Ganiraju Manyam1, Cristina Ivan, George A Calin

  • 1Department of Bioinformatics and Computational Biology, Department of Gynecologic Oncology and Reproductive Medicine and Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.

Bioinformatics (Oxford, England)
|September 10, 2013
PubMed
Summary
This summary is machine-generated.

TargetHub is a new database that integrates microRNA (miRNA)-gene interaction data. This tool simplifies the analysis of high-throughput genomic data by providing easy access to miRNA targets.

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Last Updated: May 8, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • High-throughput technologies are expanding, necessitating better understanding of genome-level data.
  • MicroRNA (miRNA) profiling is common using microarrays or next-generation sequencing.
  • Analyzing miRNA targets poses challenges due to limited tools for integrating interaction data into genomic analyses.

Purpose of the Study:

  • To present TargetHub, a novel CouchDB database for miRNA-gene interactions.
  • To provide a programmer-friendly interface for accessing miRNA target data.
  • To facilitate the integration of miRNA target interaction data into high-throughput bioinformatics analyses.

Main Methods:

  • Development of a CouchDB database to store miRNA-gene interactions.
  • Implementation of a Web site with a RESTful interface for data access.
  • Support for various gene and miRNA identifiers for versatile querying.

Main Results:

  • TargetHub provides a centralized repository of miRNA-gene interactions.
  • The database offers a programmer-friendly interface for efficient data retrieval.
  • RESTful access allows seamless integration into bioinformatics workflows.

Conclusions:

  • TargetHub serves as a valuable resource for researchers analyzing miRNA-gene interactions.
  • The tool simplifies the integration of miRNA target data into high-throughput genomic studies.
  • It enhances the utility of miRNA profiling data in various biological research areas.