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Restoring the procofactor state of factor Va-like variants by complementation with B-domain peptides

Matthew W Bunce1, Mettine H A Bos1, Sriram Krishnaswamy2

  • 1From the Division of Hematology, The Children's Hospital of Philadelphia and.

The Journal of Biological Chemistry
|September 10, 2013
PubMed
Summary

The B-domain of coagulation factor V (FV) inhibits its own activation. A basic region (BR) fragment binds to FV, blocking activation and restoring the inactive procofactor state.

Keywords:
AutoinhibitionCoagulation FactorsEnzyme MechanismsFactor VFactor VaHemostasisProtein EngineeringProthrombinaseRecombinant Protein Expression

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