Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 8, 2026

A Single-fly Assay for Foraging Behavior in Drosophila
13:01

A Single-fly Assay for Foraging Behavior in Drosophila

Published on: November 4, 2013

Insect pheromone behavior: fruit fly.

Daisuke Yamamoto1, Soh Kohatsu, Masayuki Koganezawa

  • 1Department of Developmental Biology and Neurosciences, Tohoku University Graduate School of Life Sciences, Sendai, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|September 10, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical utility of maximal and minimal intensity projections in T2-weighted MRI for neurosurgical planning.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2026
Same author

Laparoscopic Repair of an Incarcerated Parahiatal Hernia with Gastric Necrosis: A Rare Case Report.

Surgical case reports·2026
Same author

Safety and Learning Curve of Robotic Rectal Surgery Performed by Young Non-Endoscopic Surgical Skill Qualification System (ESSQS)-Qualified Surgeons: A Single-Center Retrospective Study.

Cureus·2026
Same author

Usefulness of 4K Indocyanine Green Videoangiography for Basilar Artery Identification During Endoscopic Third Ventriculostomy in Adults With Secondary Hydrocephalus: Technical Note and Four-Case Series.

Operative neurosurgery (Hagerstown, Md.)·2026
Same author

Trifluridine/tipiracil enhances radiation-induced abscopal effects and augments PD-1 blockade in gastric cancer.

Scientific reports·2026
Same author

Male Patients With β-Catenin-Positive Atypical Hepatocellular Neoplasm Arising in Inflammatory Hepatocellular Adenoma: A Case Report.

Hepatology research : the official journal of the Japan Society of Hepatology·2026

Fruitless (fru) gene activation in Drosophila melanogaster males using dTrpA1 induces courtship behaviors. Tethered male assays enable neuronal imaging during courtship stimulation, detailing these methods.

Area of Science:

  • Neurobiology
  • Animal Behavior
  • Genetics

Background:

  • Drosophila melanogaster is a key model organism for studying genetics.
  • Pheromone-guided behavior is crucial for insect reproduction.
  • The fruitless (fru) gene regulates courtship in male flies.

Purpose of the Study:

  • To detail technical methods for studying pheromone-guided courtship behavior in Drosophila.
  • To provide protocols for single-male and tethered male assays.

Main Methods:

  • Single-male assays utilize the dTrpA1 thermosensitive channel to activate fruitless (fru) expressing neurons, inducing courtship acts.
  • Tethered male assays enable calcium (Ca2+) imaging of neuronal activity during simulated courtship.
  • These assays involve stimulating male flies with females or pheromones.

More Related Videos

An Optimized Protocol for Rearing Fopius arisanus, a Parasitoid of Tephritid Fruit Flies
12:00

An Optimized Protocol for Rearing Fopius arisanus, a Parasitoid of Tephritid Fruit Flies

Published on: July 2, 2011

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults
06:47

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

Published on: June 8, 2021

Related Experiment Videos

Last Updated: May 8, 2026

A Single-fly Assay for Foraging Behavior in Drosophila
13:01

A Single-fly Assay for Foraging Behavior in Drosophila

Published on: November 4, 2013

An Optimized Protocol for Rearing Fopius arisanus, a Parasitoid of Tephritid Fruit Flies
12:00

An Optimized Protocol for Rearing Fopius arisanus, a Parasitoid of Tephritid Fruit Flies

Published on: July 2, 2011

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults
06:47

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

Published on: June 8, 2021

Main Results:

  • The described methods allow for the precise activation and observation of courtship behaviors.
  • Neuronal activity can be imaged in real-time during specific behavioral contexts.
  • These techniques facilitate the study of the genetic and neural underpinnings of courtship.

Conclusions:

  • The single-male and tethered male assays are valuable tools for neurobiological research on Drosophila courtship.
  • These detailed methods enhance the reproducibility and scope of studies on pheromone-guided behaviors.
  • Further research can leverage these assays to uncover the complex neural circuits involved in mating.