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Detection of Extracellular enzymes Activities in Various Fusarium spp
Hyuk Woo Kwon1, Ji Hwan Yoon, Seong Hwan Kim
1Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan, Chungnam 330-714, Korea.
Abstract:
Thirty seven species of Fusarium were evaluated for their ability of producing extracellular enzymes using chromogenic medium containing substrates such as starch, cellobiose, CM-cellulose, xylan, and pectin. Among the tested species Fusarium mesoamericanum, F. graminearum, F. asiaticum, and F. acuminatum showed high β-glucosidase acitivity. Xylanase activity was strongly detected in F. proliferatum and F. oxysporum. Strong pectinase activity was also found in F. oxysporum and F. proliferatum. Amylase activity was apparent in F. oxysporum. No clear activity in cellulase was found from all the Fusarium species tested.
Insights
This study evaluated 37 Fusarium species for extracellular enzyme production. Fusarium species like F. mesoamericanum and F. oxysporum showed significant activity in beta-glucosidase, xylanase, and pectinase, but not cellulase.
Area of Science:
- Microbiology
- Enzymology
- Mycology
Background:
- Fusarium species are significant plant pathogens and saprophytes.
- Extracellular enzymes play crucial roles in nutrient acquisition and pathogenicity.
- Understanding enzyme profiles aids in species differentiation and ecological role assessment.
Purpose of the Study:
- To investigate and compare the extracellular enzyme-producing capabilities of 37 Fusarium species.
- To identify specific Fusarium species with high activity in degrading starch, cellobiose, CM-cellulose, xylan, and pectin.
Main Methods:
- Utilized chromogenic media with specific substrates (starch, cellobiose, CM-cellulose, xylan, pectin).
- Evaluated 37 Fusarium species for the production of key extracellular enzymes.
- Quantified enzyme activity based on colorimetric reactions.
Main Results:
- High beta-glucosidase activity observed in Fusarium mesoamericanum, F. graminearum, F. asiaticum, and F. acuminatum.
- Strong xylanase and pectinase activity detected in Fusarium oxysporum and F. proliferatum.
- Amylase activity was apparent in F. oxysporum; no significant cellulase activity was found across tested species.
Conclusions:
- Fusarium species exhibit diverse extracellular enzyme profiles.
- Specific enzymes like beta-glucosidase, xylanase, and pectinase are differentially produced among Fusarium species.
- Enzyme activity patterns may contribute to understanding Fusarium ecological roles and pathogenicity.
