Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tresyl-activated support for high-performance affinity chromatography.

K Nakamura1, T Hashimoto, Y Kato

  • 1Central Research Laboratory, TOSOH Corporation, Yamaguchi, Japan.

Journal of Chromatography
|June 27, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Changes in maxillofacial morphology due to improvement of nasal obstruction in rats.

Orthodontics & craniofacial research·2018
Same author

Trends in psychological distress and alcoholism after The Great East Japan Earthquake of 2011.

SSM - population health·2018
Same author

Abnormal asymmetries in subcortical brain volume in schizophrenia.

Molecular psychiatry·2016
Same author

EDUCATION AND IMAGING. Gastrointestinal: Needle tract implantation after endoscopic ultrasound-guided fine-needle aspiration of a pancreatic adenocarcinoma.

Journal of gastroenterology and hepatology·2015
Same author

Sitagliptin, a dipeptidyl peptidase-4 inhibitor, increases the number of circulating CD34⁺CXCR4⁺ cells in patients with type 2 diabetes.

Endocrine·2015
Same author

Does Internet-based cognitive behavioral therapy (iCBT) prevent major depressive episode for workers? A 12-month follow-up of a randomized controlled trial.

Psychological medicine·2015
Same journal

Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash chromatography.

Journal of chromatography·2013
Same journal

[Electrophoresis of radionuclides on cellogel].

Journal of chromatography·2012
Same journal

Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.

Journal of chromatography·2010
Same journal

Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993).

Journal of chromatography·1994
Same journal

Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.

Journal of chromatography·1994
Same journal

Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.

Journal of chromatography·1994
See all related articles

A novel activated gel support, Tresyl-5PW, enables efficient antibody coupling under mild conditions. This method achieves high yields and antigen binding, facilitating rapid, non-denaturing separation of human serum proteins.

Area of Science:

  • Biochemistry
  • Materials Science
  • Immunology

Background:

  • Antibody immobilization is crucial for various biochemical assays and purification techniques.
  • Developing efficient and mild methods for antibody coupling remains a key challenge in bioseparation.

Purpose of the Study:

  • To evaluate the efficacy of a new activated support, Tresyl-5PW gel, for antibody coupling.
  • To determine optimal conditions for antibody immobilization on Tresyl-5PW.
  • To assess the performance of immobilized antibodies for antigen capture and separation.

Main Methods:

  • Coupling of antibodies to Tresyl-5PW gel support under varying conditions.
  • Optimization of reaction time, temperature, and buffer concentration (1 M phosphate buffer, pH 7.5).

Related Experiment Videos

  • Evaluation of antibody coupling yield and antigen binding efficiency using human serum proteins.

    • Main Results:

    • High antibody coupling yields (>80%) achieved under mild conditions (25°C, 1-2 M phosphate buffer).
    • Optimal coupling achieved with 2-3 mg/mL antibody (2h) or 10 mg/mL antibody (6-7h).
    • Antigen binding efficiencies ranged from 70-80%, suggesting selective Fc region attachment.
    • Rapid, non-denaturing separation of human serum proteins demonstrated.

    Conclusions:

    • Tresyl-5PW is an effective activated support for antibody immobilization.
    • The developed coupling method is efficient, mild, and yields high-performing antibody conjugates.
    • This system is suitable for rapid and stable separation of antigens like human serum proteins.