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Related Experiment Video

Updated: May 8, 2026

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

Anchoring foreign substances on live cell surfaces using Sortase A specific binding peptide.

Kyoungsook Park¹, Juyeon Jung, Jieun Son

¹BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Major of Nanobiotechnology, University of Science and Technology, 125 Gwahangno, Yuseong, Daejeon 305-806, Republic of Korea.

Chemical Communications (Cambridge, England)

|September 11, 2013

Summary

A new live cell surface labeling method uses Sortase A enzyme and a specific binding peptide for direct cell conjugation. This technique offers a simple, rapid, and efficient way to label cells in real-time.

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Published on: April 9, 2018

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Last Updated: May 8, 2026

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

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Published on: January 17, 2019

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Area of Science:

Biotechnology
Cell Biology
Molecular Biology

Background:

Cell surface labeling is crucial for various biological studies.
Existing methods can be complex or time-consuming.

Purpose of the Study:

To develop a simple, rapid, and efficient live cell surface labeling method.

Main Methods:

Utilized transient expression of Sortase A at the cell surface.
Employed direct conjugation with a Sortase A specific binding peptide.

Main Results:

Successfully achieved live cell surface labeling.
Demonstrated a simple, rapid, and efficient labeling process.

Conclusions:

The developed method provides an effective approach for live cell surface labeling.
This technique has potential applications in cell tracking and analysis.