Related Experiment Video
Updated: May 8, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
Mineral content in teeth with deciduous molar hypomineralisation (DMH)
M E C Elfrink1, J M ten Cate, L J van Ruijven
1Department of Cariology, Endodontology and Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.
Yellow or brown opacities in molar hypomineralisation (DMH) indicate significantly lower enamel mineral density. White opacities show no difference, highlighting the need for preventive strategies in DMH management.
Area of Science:
- Dental research
- Mineralogy
- Pediatric dentistry
Background:
- Molar incisor hypomineralisation (DMH) affects enamel quality.
- Understanding mineral density variations in DMH opacities is crucial for diagnosis and treatment.
Purpose of the Study:
- To quantify and compare the hydroxyapatite mineral density of sound and opaque areas in DMH molars.
- To use sound deciduous teeth as controls for comparison.
Main Methods:
- Micro-computed tomography (microCT) scanning of 29 extracted primary molars.
- Analysis of teeth with yellow, white, and brown opacities, and sound molars.
- Statistical analysis using ANOVA and t-tests to determine significant differences.
Main Results:
- Yellow and brown opacities showed significantly lower mineral density (1368-1407 mg HA/cm²) compared to sound enamel (1747-1758 mg HA/cm²).
- White opacities had similar mineral density (1737 mg HA/cm²) to sound enamel.
- Mineral density in yellow and brown opacities was intermediate between dentine and enamel.
Conclusions:
- DMH molars with yellow or brown opacities exhibit a 20-22% reduction in enamel mineral density.
- White opacities do not correlate with reduced mineral content.
- The findings underscore the importance of a preventive approach for DMH due to compromised enamel integrity.
Related Concept Videos
Essential Minerals for Bone Health
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin and...
Tooth Anatomy
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or grinding food.
Minerals
Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
Oral Cavity
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...

