Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lynch syndrome caused by a pathogenic SINE-VNTR-Alu (SVA) insertion in MSH2 gene identified by long-read DNA sequencing.

Familial cancer·2026
Same author

Early-life nutritional environment is associated with late-life cognition in the Health and Retirement Study, a pellagra epidemic natural experiment.

medRxiv : the preprint server for health sciences·2026
Same author

Gut bacterial metabolite imidazole propionate potentiates Alzheimer's disease pathology.

Nature communications·2026
Same author

Evaluating statistical models for overdispersed multi-omics data: a multiplex immunofluorescence case study.

American journal of epidemiology·2026
Same author

<i>TGFBR1</i>*6A and Risk for Colorectal Cancer.

Cancer communications (London, England)·2026
Same author

Genotoxic pks + E. coli is strongly associated with ileocolonic neoplasia in adults with Cystic Fibrosis.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2026

Related Experiment Video

Updated: May 8, 2026

Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Telomere length varies by DNA extraction method: implications for epidemiologic research.

Julie M Cunningham1, Ruth A Johnson, Kristin Litzelman

  • 1Authors' Affiliations: Departments of Laboratory Medicine and Pathology, Health Sciences Research, and Gastroenterology, Mayo Clinic College of Medicine; Mayo Clinic Cancer Center, Biomedical Statistics and Informatics, Mayo Clinic College of Medicine, Rochester, Minnesota; Departments of Population Health Sciences, and Biostatistics and Medical Informatics, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin; University of Michigan, Ann Arbor, Michigan; Division of Gastroenterology, Mayo Clinic, Jacksonville, Florida; Biostatistics & Epidemiology, Geisel School of Medicine, Dartmouth University, Hanover, New Hampshire; Public Health Sciences Division, Cancer Prevention Program, Fred Hutchinson Cancer Research Center, Seattle, Washington; The Stanford Cancer Institute and Stanford School of Medicine, Department of Medicine, Stanford, California; Queensland Institute of Medical Research, Clive Berghofer Cancer Research Centre, Brisbane, Queensland; and Melbourne School of Population Health, The University of Melbourne, Parkville, Victoria, Australia.

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
|September 11, 2013
PubMed
Summary

DNA extraction methods significantly impact telomere length measurements in colorectal cancer studies. QIAamp yielded shorter telomere lengths compared to PureGene or phenol/chloroform, affecting cancer risk associations.

More Related Videos

Monochrome Multiplex Quantitative PCR Telomere Length Measurement
11:44

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Published on: March 22, 2024

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

Related Experiment Videos

Last Updated: May 8, 2026

Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Monochrome Multiplex Quantitative PCR Telomere Length Measurement
11:44

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Published on: March 22, 2024

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Telomere length in peripheral blood leukocyte (PBL) DNA is linked to cancer risk, but findings are inconsistent.
  • Discrepancies may arise from variations in DNA preparation methods.
  • This study investigates DNA extraction's effect on telomere length in colorectal cancer.

Purpose of the Study:

  • To compare the impact of three DNA extraction methods on relative telomere length (RTL) measurements.
  • To assess how these variations influence the interpretation of telomere length in colorectal cancer research.

Main Methods:

  • Quantitative PCR (qPCR) was used to measure PBL relative telomere length (RTL).
  • DNA was extracted from 1,033 colorectal cancer patients and 2,952 controls using phenol/chloroform, PureGene, or QIAamp methods.
  • An independent validation set of 44 individuals was used to confirm findings.

Main Results:

  • Significant differences in RTL were observed based on DNA extraction method (P < 0.001).
  • QIAamp extraction resulted in lower mean RTL (0.38) compared to PureGene (0.75) and phenol/chloroform (0.78).
  • qPCR measurements from QIAamp-extracted DNA showed a narrower range (0.17-0.58) than the other methods.

Conclusions:

  • DNA extraction method significantly influences RTL measurements obtained by qPCR.
  • These method-dependent variations can explain inconsistencies in studies linking telomere length to cancer risk.
  • Standardizing DNA extraction protocols is crucial for reliable telomere length analysis in disease research.