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Updated: May 8, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
One-electron oxidation of antioxidants: a kinetic-thermodynamic correlation
Laura S Villata1, Andrea M Berkovic, Mónica C Gonzalez
1Universidad Nacional de La Plata, Diag 113 y 64, La Plata, Argentina.
Abstract:
The values of the bimolecular rate constants for the reactions of 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radical cation with epicatechin (((2.4 ± 0.2)) s(-1) M(-1)), and epigallocatechingallate ((29 ± 5) s(-1) M(-1)) were obtained by spectrophotometric measurements. We propose a correlation between the Gibbs energy ΔG(o) for the one-electron charge-transfer reactions from several antioxidants to radical species and the rate constants of the corresponding bimolecular reactions. This correlation can be used to predict rate constants of reactions of known ΔG(o) values.
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