Effect of storage on blood count variables in small samples

Area of Science:

• Clinical Hematology
• Laboratory Medicine
• Sample Stability Studies

Background:

• Accurate hematological analysis is crucial for patient diagnosis and monitoring.
• The reliability of small-volume blood samples (micro-samples) for mailed transport and storage requires investigation.
• Understanding sample stability is essential for maintaining diagnostic integrity.

Purpose of the Study:

• To evaluate the stability of various hematological parameters in micro-samples after mail transport and storage.
• To compare the reliability of micro-samples with larger venous blood samples under different storage conditions.
• To determine the clinical suitability of micro-samples for routine hematological testing.

Main Methods:

• Analysis of 186 micro-samples from venous and skin puncture blood.
• Storage of samples at room temperature and 4°C for up to 72 hours.
• Measurement of key hematological variables including white cell, red cell, hemoglobin, MCH, platelet, haematocrit, MCV, and MCHC.

Main Results:

• White cell, red cell, hemoglobin, MCH, and platelet counts remained stable for clinical purposes.
• Haematocrit, MCV, and MCHC values showed instability when samples were stored at room temperature.
• Micro-samples demonstrated comparable reliability to larger venous blood volumes for most critical hematological variables.

Conclusions:

• Micro-samples are a reliable option for mailed transport and storage for essential hematological tests.
• Careful consideration of storage conditions is necessary, particularly for parameters like haematocrit and red blood cell indices.
• The findings support the use of micro-samples without compromising the reliability of crucial hematological assessments.