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Updated: May 8, 2026

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
Covalent modification of cell surfaces with TLR agonists improves & directs immune stimulation
Janine K Tom1, Rock J Mancini, Aaron P Esser-Kahn
1Department of Chemistry, University of California Irvine, Irvine, CA 92697, USA. aesserka@uci.edu.
Abstract:
We present a primary example of a cell surface modified with a synergistic combination of agonists to tune immune stimulation. A model cell line, Lewis Lung Carcinoma, was covalently modified with CpG-oligonucleotides and lipoteichoic acid, both Toll-like receptor (TLR) agonists. The immune-stimulating constructs provided greater stimulation of NF-κB in a model cell line and bone marrow-derived dendritic cells than the components unconjugated in solution.
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