Related Experiment Video
Updated: May 8, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
E6/E7 mRNA expression of high-risk HPV types in 849 Greek women
Elena Argyri1, Elpida Tsimplaki, Dimitra Daskalopoulou
1Department of Virology, St. Savvas, Regional Anticancer Oncology Hospital of Athens, 171 Alexandras Avenue, 11522 Athens, Greece. +30 2106409461, epanot@tee.gr.
Aim:
The goal of this study was to investigate human papillomavirus (HPV) geno typing and high-risk HPV E6/E7 mRNA expression in 849 women.
Patients And Methods:
849 Cervical samples were obtained from patients attending an outpatient clinic to have their annual gynaecological check-up. All patients underwent a conventional Pap test. The patients were also offered HPV test with the knowledge that it is not part of the screening.
Results:
Overall prevalence of HPV DNA was 41.3%, while E6/E7 mRNA expression was found in 20.7% of the patients. HPV DNA and E6/E7 mRNA expression were detected in 21.1% and 9.1% of normal cytological samples, respectively, 38.1% and 23.8% of atypical squamous cells of unknown significance, 84.8% and 40.7% of low-grade squamous intraepithelial lesions, and 96.4% and 89.3% of high-grade squamous intraepithelial lesions, respectively. HPV 16 was the most frequent genotype identified.
Conclusion:
E6/E7mRNA detection might be useful as a screening marker for the early prediction of active infections and subsequent progression to severe dysplasia.
