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Updated: May 8, 2026
Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
[Development of Clostridium perfringens selective chromogenic medium]
Jing Wang1, Ping Chen, Qianqian Li
1College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China. tracy_87911@163.com
Objective:
To screen the Clostridium perfringens selective composition to develop a Clostridium perfringens selective chromogenic media.
Methods:
To evaluate the role in promoting the growth of the target bacteria of growth factor such as mannitol, sodium pyruvate, and magnesium sulfate. Comparing the inhibition of antibiotics such as cycloserine, neomycin, polymyxin and sulfadiazine of target bacteria and non-target bacteria. To compare the reaction of chromogenic substrates such as BCIP, PNPP, X-Gal, Mu-Gal and ONPG. Screening the best enzymatic factors among magnesium sulfate, calcium sulfate, manganese sulfate, zinc sulfate. Then to determine the optimal dose. To determine the ultimate composition of chromogenic media.
Results:
Ultimately determines the composition of media, sodium pyruvate 200 mg, cycloserine 0.5 mg, BCIP 6 mg, Mu-Gal 6 mg, magnesium sulfate 72 mg. Add all the composition into 100 mL nutritional broth medium to prepare the medium. Clostridium perfringens growth in chromogenic medium, TSC medium and SPS medium have no significant difference.
Conclusion:
Clostridium perfringens selective chromogenic medium can be used in detection of Clostridium perfringens.
