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Updated: May 8, 2026
Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
Published on: June 28, 2024
[Detection of Salmonella, Shigella and Staphylococcus aureus based on quantum dots and immunomagnetic beads]
Qianqian Li1, Ping Chen, Jing Wang
1College of Food Science and Engineering, Jilin Agriculture University, Changchun 130118, China. lqq_1986@163.com
Objective:
To establish a detecting method of Salmonella, Shigella and Staphylococcus aureus by using quantum dots and immunomagnetic beads.
Methods:
The polychrome colour CdSe quantum dots were synthetized by water phase process. Triad colour quantum dots were conjugated with specific anti-Salmonella, anti-Shigella and anti-Staphylococcus aureus antibody by covalent cross-linking reaction, and then the coupled product was confirmed by SDS-PAGE and ultraviolet absorption spectrum. Using immunomagnetic enrichment and quantum dots labeling specific antibody, the fluorescence intensity of Salmonella, Shigella and Staphylococcus aureus were observed by fluorescence microscope.
Results:
The coupled reaction was successful by SDS-PAGE and ultraviolet absorption spectrum. Using immunomagnetic enrichment and quantum dots with different color and different wavelength labeling specific antibody, the detection time was less than 2h in the same reaction system. The samples infection with target bacteria were detected directly. The detection limit was 10 CFU/ml.
Conclusion:
This method can detect Salmonella, Shigella and Staphylococcus aureus quickly and specificly.
