Related Experiment Video
Updated: May 8, 2026
Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
Published on: July 14, 2023
[Effect comparison between two ELISA kits in IgG antibody detection of Echinococcus granulosus]
Yan-Hong Chu1, Yu-Chun Cai, Lin Ai
1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center of Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Objective:
To compare the effects of two ELISA kits on IgG antibody detection of human Echinococcus granulosus.
Methods:
A Total of 134 sera of patients with echinococcosis, paragonimiasis westermani, clonorchiasis sinensis, schistosomiasis japonica, and cysticercosis cellulosae, and normal persons were detected by two IgG ELISA kits produced by different companies. Furthermore, the specificity, sensitivity and cross reactivity were counted and analyzed statistically.
Results:
The sensitivity and specificity were extremely high of the two kits as 100.00%. The cross-reactivity rates were 25.00% (paragonimiasis westermani), 26.09% (clonorchiasis sinensis), 10.00% (schistosomiasis japonica), and 87.5% (cysticercosis), respectively, by using the kit produced by the Combined Company in Shenzhen; the cross-reactivity rates were 5.00% (paragonimiasis westermani), 13.04% (clonorchiasis sinensis), 20.00% (schistosomiasis japonica), and 93.75% (cysticercosis) respectively, by using the kit produced by Haitai Company in Zhuhai. In addition, there was a significant difference of Paragonimus westermani detection (P < 0.05), but the rests had no statistically significant differences (all P > 0.05) between the two kits.
Conclusion:
Both ELISA kits on IgG antibody detection of human Echinococcus granulosus have the advantages of a high sensitivity, specificity, convenience and high-speed. However, it is also in urgent need to further solve the cross-reactivity of Echinococcus granulosus with other parasites, in order to improve the accuracy of early diagnosis.
