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Related Experiment Video

Updated: May 8, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Transcriptomic characterization of cold acclimation in larval zebrafish.

Yong Long1, Guili Song, Junjun Yan

  • 1The Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, PR China. zbcui@ihb.ac.cn.

BMC Genomics
|September 13, 2013
PubMed
Summary

Zebrafish larvae develop cold tolerance through transcriptional and post-transcriptional changes. Mild cold exposure enhances survival, involving altered gene expression, splicing, and ribosome biogenesis.

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Area of Science:

  • Developmental Biology
  • Environmental Science
  • Genomics

Background:

  • Temperature significantly impacts fish life cycles, necessitating understanding cold acclimation mechanisms.
  • Fish larvae's adaptation to cold stress and associated transcriptional changes are poorly understood.

Purpose of the Study:

  • To characterize cold tolerance development in zebrafish larvae.
  • To investigate cold-specific transcriptional alterations in zebrafish larvae using RNA-sequencing.

Main Methods:

  • Zebrafish larvae (96 hours post fertilization) were exposed to mild cold stress (16°C) followed by severe cold stress (12°C).
  • RNA-sequencing (RNA-seq) was employed to analyze transcriptional profiles under cold stress.
  • Differential gene expression, gene ontology enrichment, and alternative splicing analyses were performed.

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Last Updated: May 8, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
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Main Results:

  • Pre-exposure to mild cold significantly increased zebrafish larvae survival rates under severe cold.
  • RNA-seq identified 1,431 up-regulated and 399 down-regulated genes.
  • Cold stress induced significant changes in RNA splicing, ribosome biogenesis, protein catabolism, and RNA transport, including alternative splicing and promoter switching.

Conclusions:

  • Zebrafish larvae can develop cold tolerance via mild low-temperature acclimation.
  • Transcriptional and post-transcriptional regulations play crucial roles in this cold acclimation process.