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Updated: May 8, 2026
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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Mycobacterium tuberculosis detection in blood using multiplex nested polymerase chain reaction.
1Molecular Division, Research & Development Centre, Kilpest India Ltd, Bhopal, India.
Summary
The Blacklight Card standardizes polymerase chain reaction (PCR) for tuberculosis (TB) diagnosis in blood samples. This method shows high sensitivity and specificity, aiding in rapid diagnosis of difficult TB cases.
Area of Science:
- Microbiology
- Molecular Diagnostics
- Infectious Diseases
Background:
- Polymerase chain reaction (PCR) is a promising tool for rapid, sensitive, and specific tuberculosis (TB) diagnosis.
- Standardization of blood-based TB PCR remains a challenge for clinical application.
Purpose of the Study:
- To evaluate the Blacklight Card for standardizing TB PCR in blood samples.
- To assess its utility as an auxiliary diagnostic tool for TB using a multiplex nested PCR method.
Main Methods:
- The Blacklight Card was standardized as a novel extraction tool.
- A multiplex nested PCR assay targeting insertion sequence 6110 and MPB64 was employed.
- 130 blood samples from patients with clinical TB symptoms were analyzed.
Main Results:
- PCR detected Mycobacterium tuberculosis DNA in 22 out of 130 samples (17%).
- The assay demonstrated high diagnostic accuracy with 95.7% sensitivity and 100% specificity.
- Positive and negative predictive values were 85.1% and 99.2%, respectively.
Conclusions:
- The Blacklight Card shows potential as a valuable tool for the rapid diagnosis of TB.
- It may be particularly useful for disseminated and extra-pulmonary TB forms.
- This method can aid in urgent diagnoses for severely ill patients.
