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Development of a membrane-based microwave-mediated electrochemical ELISA method for TNF-α detection in patients with
Irmina Diala1, Shinobu Sato, Michihiko Usui
1Research Center for Bio-microsensing Technology, Kyushu Institute of Technology.
Abstract:
The tumor necrosis factor-α (TNF-α) is implicated in periodontal disease, and there was an attempt to quantitate it by a membrane-based microwave-mediated electrochemical enzyme-linked immunosorbent assay (MMeELISA) using p-aminophenyl phosphate (pAPP) with an over-all time of 1.5 h. A differential pulse voltammetric signal increased linearly with an increase in the amount of TNF-α with a detection limit of 0.48 pg mL(-1). This bio-sensing platform revealed a significant difference in the TNF-α level between GCF samples from periodontal patients and healthy volunteers.