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Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts

Naoko Kurebayashi1, Mari Sato, Toshiaki Fujisawa

  • 1Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan; Department of Dental Anesthesiology, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan.

Biochemical and Biophysical Research Communications
|September 13, 2013
PubMed

Insights

Bone morphogenetic protein 2 (BMP2) signaling regulates Y1 receptor expression in osteoblasts. Blocking the Y1 receptor increases markers of bone formation, suggesting an autocrine role for neuropeptide Y.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Endocrinology

Background:

  • The neuropeptide Y (NPY) system is a key neural pathway involved in various physiological processes.
  • The NPY-Y1 receptor axis plays a role in bone homeostasis, as indicated by high bone mass in osteoblast-specific Y1 receptor knockout mice.
  • Regulation of Y1 receptor expression during osteoblast differentiation is not well understood.

Purpose of the Study:

  • To investigate the role of bone morphogenetic protein (BMP) 2 signaling in regulating Y1 receptor expression in osteoblasts.
  • To explore the potential autocrine mechanism of NPY action in osteoblasts.

Main Methods:

  • Utilized C2C12 and MC3T3-E1 cell lines.
  • Investigated Y1 receptor mRNA expression following BMP2 treatment.
  • Performed co-transfection assays with Smad1 and Smad4.
  • Analyzed transcriptional activity via Y1 receptor gene promoter interaction.
  • Used small interfering RNA (siRNA) to knock down Y1 receptor expression.

Main Results:

  • BMP2 treatment induced Y1 receptor mRNA expression in C2C12 cells.
  • Co-transfection with Smad1 and Smad4 mimicked BMP2-induced Y1 receptor expression.
  • Smad1/4 enhanced transcriptional activity of the Y1 receptor gene promoter.
  • siRNA-mediated knockdown of Y1 receptor in MC3T3-E1 cells increased alkaline phosphatase, osteocalcin, Runx2, and osterix expression.

Conclusions:

  • BMP2 signaling is a key regulator of Y1 receptor gene expression in osteoblasts.
  • NPY may act on osteoblasts via an autocrine mechanism.
  • Y1 receptor signaling might negatively regulate osteoblast differentiation markers.
Keywords:
Bone morphogenetic protein 2Neuropeptide YOsteoblastsY1 receptor

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