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Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
Published on: February 24, 2014
Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions
Sandra S Richter1, Kristopher P Heilmann, Cassie L Dohrn
1Cleveland Clinic, Cleveland, Ohio, USA.
Journal of Clinical Microbiology
|September 13, 2013
Abstract:
Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach.
