Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Endometriosis of the spleen - multidisciplinary surgical solution in a patient with a complex picture of deep infiltrating pelvic endometriosis.

Ceska gynekologie·2026

Same author

Right-sided hemicolectomy with complete mesocolic excision and modified D3 lymphadenectomy - a pilot study.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2026

Same author

Commentary on the CheckMate 9ER clinical trial.

Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti·2025

Same author

Different Measures of Hyperglycemia Are Negatively Associated With Skin Microvascular Flowmotion: The Maastricht Study.

Microcirculation (New York, N.Y. : 1994)·2024

Same author

The complex polyploid genome architecture of sugarcane.

Nature·2024

Same author

Enzalutamide and abiraterone in the treatment of patients with metastatic castration-resistant prostate cancer treated previously with chemotherapy.

Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti·2024

Search research articles

Related Experiment Video

Updated: May 8, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 5, 2014

Mapping nonrecombining regions in barley using multicolor FISH.

M Karafiátová¹, J Bartoš, D Kopecký

¹Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc-Holice, Czech Republic.

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology

|September 13, 2013

Summary

This summary is machine-generated.

This study presents an efficient fluorescence in situ hybridization (FISH) technique for precisely mapping single-copy genes in plants. This method overcomes challenges in large genomes, improving physical mapping accuracy where genetic maps lack resolution.

More Related Videos

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

BEST: Barcode Enabled Sequencing of Tetrads

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Related Experiment Videos

Last Updated: May 8, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 5, 2014

Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

BEST: Barcode Enabled Sequencing of Tetrads

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

Area of Science:

Genetics
Molecular Biology
Plant Science

Background:

Fluorescence in situ hybridization (FISH) is crucial for physical mapping and ordering DNA clones like bacterial artificial chromosomes (BACs).
Mapping large genomes is challenging due to repetitive DNA, making single-copy sequence mapping (e.g., complementary DNA or cDNA) an attractive alternative.
Localizing small single-copy sequences (<10 kb) in plants using FISH remains difficult.

Purpose of the Study:

To develop and demonstrate a highly efficient FISH technique for unambiguous localization of single-copy genes in plants.
To validate the technique's utility in mapping complementary DNA (cDNA) clones in barley, particularly in challenging genomic regions.

Main Methods:

Developed a novel, highly efficient fluorescence in situ hybridization (FISH) protocol.
Applied the FISH technique to map 15 full-length complementary DNA (cDNA) clones to barley chromosome 7H.
Analyzed hybridization patterns to confirm clone order and physical locations.

Main Results:

Successfully mapped 13 out of 15 cDNA clones, demonstrating the technique's high efficiency.
Physical mapping revealed that a small genetic distance (1.2 cM) corresponded to a large physical region (>40%) of chromosome 7H.
Identified partially homologous sequences across chromosomes and refined the physical order of several cDNA clones compared to existing genetic maps.

Conclusions:

The developed FISH technique enables precise physical mapping of single-copy genes in plants, even in complex genomes.
This approach is vital for resolving marker order in genomic regions where genetic mapping is limited.
The findings highlight the importance of FISH for accurate physical mapping and understanding genome structure.