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Related Experiment Video

Updated: May 8, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression.

Yuri Tanaka¹, Yoshimi Oshima, Tomomichi Yamamura

¹Central Research Laboratories, Hokko Chemical Industry Co., Ltd, 2165 Toda, Atsugi, Kanagawa 243-0023, Japan.

Scientific Reports

|September 13, 2013

Summary

Genetic engineering using chimeric repressors controlled petal number in Cyclamen persicum (cyclamen). Modifying AGAMOUS (AG) genes CpAG1 and CpAG2 created multi-petal flowers, valuable for ornamental varieties.

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Area of Science:

Plant molecular biology
Developmental genetics
Horticultural science

Background:

Cyclamen persicum (cyclamen) is a valuable ornamental plant known for its winter blooms.
The AGAMOUS (AG) gene plays a crucial role in flower development, including stamen and carpel formation.
Understanding AG orthologues in cyclamen can unlock new horticultural traits.

Purpose of the Study:

To clone and characterize cyclamen orthologues of the AGAMOUS gene, CpAG1 and CpAG2.
To investigate the function of CpAG1 and CpAG2 in flower organ development using chimeric repressors.
To explore the potential for genetic engineering to control petal number in cyclamen for ornamental purposes.

Main Methods:

Cloning of two cyclamen AGAMOUS orthologues, CpAG1 and CpAG2.
Expression analysis of CpAG1 and CpAG2 in different floral organs.
Genetic engineering of cyclamen using chimeric repressors (CpAG1-SRDX, CpAG2-SRDX) to alter gene function.
Analysis of floral morphology in transgenic cyclamen and Arabidopsis thaliana.

Main Results:

CpAG1 and CpAG2 were cloned and found to be primarily expressed in stamens and carpels, respectively.
Repression of CpAG1 led to stamen-to-petal conversion, increasing petal number to 10.
Simultaneous repression of both CpAG1 and CpAG2 induced rose-like, multi-petal flowers, a desirable ornamental trait.
Expression of repressors in Arabidopsis thaliana confirmed similar effects on flower organ specification.

Conclusions:

The number of petals in cyclamen can be controlled through targeted genetic engineering of AG orthologues using chimeric repressors.
This strategy offers a potential method for developing novel multi-petal ornamental varieties of cyclamen and possibly other plant species.
The distinct roles of CpAG1 and CpAG2 provide a basis for precise manipulation of floral architecture.