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Updated: May 8, 2026
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and
Fang Liang1, Neetika Arora, Kang Liang Zhang
1Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, QLD, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|September 13, 2013
Summary
A new quantitative real-time PCR (qPCR) method, PrimRglo™, enables simultaneous detection and quantification of multiple viruses or bacteria. This sensitive technology offers a powerful tool for nucleic acid detection and diagnostics.
Area of Science:
- Molecular Biology
- Biotechnology
- Infectious Disease Diagnostics
Background:
- Quantitative real-time polymerase chain reaction (qPCR) is a crucial tool for accurate nucleic acid detection.
- Existing qPCR methods face limitations in multiplexing capacity and simultaneous quantification of multiple targets.
Purpose of the Study:
- To develop a novel real-time multiplex quantitative PCR (qPCR) technology, PrimRglo™.
- To enable high-degree multiplexing for simultaneous detection and quantification of multiple nucleic acid targets.
Main Methods:
- Development of PrimRglo™ technology utilizing oligonucleotide-tagged PCR primers.
- Integration of complementary fluorophore-labelled and quencher-labelled oligonucleotides.
- Comparison of PrimRglo™ analytic sensitivity against Taqman® and SYBR green detection systems.
Main Results:
- PrimRglo™ technology demonstrated potential for high-degree multiplexing in a single reaction tube.
- The system allows for simultaneous detection and quantification of viral or bacterial load.
- Analytic sensitivity was evaluated in comparison to established qPCR detection systems.
Conclusions:
- PrimRglo™ represents a novel advancement in real-time multiplex qPCR technology.
- The technology offers enhanced capabilities for detecting and quantifying multiple nucleic acid targets simultaneously.
- PrimRglo™ has significant potential as an investigative and diagnostic tool.
