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Updated: May 8, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

High-throughput functional microRNA profiling using recombinant AAV-based microRNA sensor arrays.

Wenhong Tian¹, Xiaoyan Dong, Xiaobing Wu

¹College of Life Science, Jilin University, Changchun, Jilin, China.

Methods in Molecular Biology (Clifton, N.J.)

|September 13, 2013

Summary

High-throughput functional microRNA (miRNA) profiling is limited. This chapter introduces a novel recombinant adeno-associated virus-based miRNA sensor array for efficient miRNA profiling in cultured cells.

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Last Updated: May 8, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

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Published on: December 3, 2010

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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Published on: April 14, 2015

Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

Functional microRNA (miRNA) profiling is crucial for understanding cellular processes.
Existing methods for miRNA profiling lack high-throughput capabilities.
There is a need for efficient and scalable tools to assess miRNA function.

Purpose of the Study:

To describe a novel method for high-throughput functional miRNA profiling.
To introduce a recombinant adeno-associated virus-based miRNA sensor array (miRNA Asensor array).
To detail the preparation and application of the miRNA Asensor array for cultured cells.

Main Methods:

Development of a recombinant adeno-associated virus (AAV) vector system.
Construction of a miRNA sensor array (miRNA Asensor array) utilizing AAV vectors.
Application of the miRNA Asensor array for profiling functional miRNAs in cell cultures.

Main Results:

The miRNA Asensor array enables high-throughput functional miRNA profiling.
The method is applicable to assessing miRNA activity in cultured cells.
Detailed protocols for array preparation and usage are provided.

Conclusions:

The developed miRNA Asensor array addresses the need for high-throughput functional miRNA profiling.
This AAV-based system offers a valuable tool for miRNA research in cell-based assays.
The chapter provides a comprehensive guide to implementing this novel profiling technique.