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Related Experiment Video

Updated: May 8, 2026

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Expulsion of micronuclei containing amplified genes contributes to a decrease in double minute chromosomes from

Wei Ji¹, Zehua Bian, Yang Yu

¹Laboratory of Medical Genetics, Harbin Medical University, Harbin, People's Republic of China.

International Journal of Cancer

|September 13, 2013

Summary

Suppressing double minute chromosome (DM)-carried genes reduces DM formation and amplification in tumor cells. This process involves DNA damage and leads to decreased cell proliferation and invasion.

Keywords:

double minute chromosomes gene amplification micronuclei tumor

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An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

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Published on: May 13, 2019

The Micronucleus Assay on Cryopreserved Whole Blood

The Micronucleus Assay on Cryopreserved Whole Blood

Published on: February 23, 2024

Area of Science:

Oncology
Genetics
Molecular Biology

Background:

Double minute chromosomes (DMs) are associated with gene amplification in cancer.
The precise relationship between DM formation and the amplification of genes they carry is not fully understood.

Purpose of the Study:

To investigate the role of DM-carried genes in DM formation and amplification.
To explore the impact of downregulating DM-carried genes on tumor cell behavior.

Main Methods:

Analysis of gene amplification in NCI-H716 and SK-PN-DW cell lines using Affymetrix SNP Array 6.0.
RNA interference to downregulate seven specific DM-carried genes.
Assessment of DM formation, gene amplification, DNA damage (γH2AX foci), cell proliferation, and invasion.

Main Results:

Downregulation of DM-carried genes decreased DM numbers and their amplification via micronuclear expulsion.
Increased γH2AX foci indicated a link between DNA damage and DM loss.
Reduced DM amplification and expression of DM-carried genes correlated with decreased cell proliferation and invasion.

Conclusions:

DM-carried genes play a crucial role in the maintenance and amplification of DMs.
Targeting these genes can lead to DM loss, DNA damage, and impaired tumor cell growth and metastasis.