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Published on: November 3, 2018
Hexavalent Chromate Reductase Activity in Cell Free Extracts of Penicillium sp
Damaris L Arévalo-Rangel1, Juan F Cárdenas-González, Víctor M Martínez-Juárez
1Universidad Autónoma de San Luis Potosí, Facultad de Ciencias Químicas, Centro de Investigación y de Estudios de Posgrado, Laboratorio de Micología Experimental Avendia Dr. Manuel Nava No. 6, Zona Universitaria, 78320 San Luis Potosí, SLP, Mexico.
Abstract:
A chromium-resistant fungus isolated from contaminated air with industrial vapors can be used for reducing toxic Cr(VI) to Cr(III). This study analyzes in vitro reduction of hexavalent chromium using cell free extract(s) of the fungus that was characterized based on optimal temperature, pH, use of electron donors, metal ions and initial Cr(VI) concentration in the reaction mixture. This showed the highest activity at 37°C and pH 7.0; there is an increase in Cr(VI) reductase activity with addition of NADH as an electron donor, and it was highly inhibited by Hg(2+), Ca(2+) and Mg(2+), and azide, EDTA, and KCN.
