[Isolation, screening and identification of prometryne-degrading bacteria and their degrading characteristics]

The purpose of this study was to develop some bacterial strains that could degrade prometryne effectively. Bacteria were screened and purified from sediments of a pesticide plant with the method of domestication with gradient concentrations. Two strains were isolated and named P-1 and P-2, respectively. According to their morphology, physiological, biochemical properties and the analysis of their 16S rRNA gene sequences, the strain P-1 is Gram negative and identified as Ochrobactrum; the strain P-2 is Gram positive and identified as Bacillus. GenBank Accession numbers are HM004554 and HM004555, respectively. Degradation rates of the strains P-1 and P-2 for prometryne concentration with 40 mg x L(-1) were 46.5% and 65.4% after 12 days in the liquid culture experiment, respectively. This study provides an essential material and theoretical foundation for the remediation of prometryne contaminated soils.