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[Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp. 40027]

Xumei Geng1, Xin Chen, Xiaotong Yang

  • 1Key Lab for Biotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan 430074, China. gengxumei@163.com

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|September 14, 2013
PubMed
Abstract

Insights

The replication region of the Micromonospora sp. plasmid pJTU112 was identified within a 4.7 kb DNA fragment. This region contains five open reading frames, some related to plasmid conjugation.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Context:

  • Micromonospora sp. 40027 produces the antibiotic fortimicin A.
  • The bacterium harbors two plasmids, pJTU101 and pJTU112.
  • Understanding plasmid replication is crucial for genetic manipulation.

Purpose:

  • To clone and sequence the replication region of plasmid pJTU112.
  • To analyze the sequence of the pJTU112 replication region.

Summary:

  • The replication region of plasmid pJTU112 was localized to a 4.7 kb SacI-KpnI DNA fragment.
  • This fragment was cloned and introduced into Micromonospora sp. LXH20 via conjugation.
  • Sequence analysis revealed five open reading frames (ORFs) within the 4.7 kb fragment.
  • ORFs pJTU112.1 and pJTU112.2, along with pJTU112.3, pJTU112.4, and pJTU112.5, are associated with plasmid conjugation.

Impact:

  • Identifies the minimal DNA fragment required for pJTU112 replication.
  • Provides insights into the genetic elements governing plasmid maintenance and transfer in Micromonospora.
  • Facilitates future genetic engineering of Micromonospora for antibiotic production or other applications.

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