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Related Experiment Video

Updated: May 7, 2026

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

MiR-9 reduces human acyl-coenzyme A:cholesterol acyltransferase-1 to decrease THP-1 macrophage-derived foam cell

Jiajia Xu¹, Guangjing Hu, Ming Lu

¹State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Acta Biochimica Et Biophysica Sinica

|September 14, 2013

Summary

MicroRNA-9 (miR-9) targets ACAT1 mRNA, reducing cholesterol ester accumulation and foam cell formation. This suggests miR-9 plays a role in cholesterol homeostasis and atherosclerosis development.

Keywords:

ACAT1 atherosclerosis cholesteryl ester foam cell microRNA

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Last Updated: May 7, 2026

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

Area of Science:

Molecular Biology
Biochemistry
Cardiovascular Research

Background:

MicroRNAs (miRNAs) are key regulators of gene expression impacting various biological processes.
Dysregulation of lipid and cholesterol metabolism is implicated in diseases like atherosclerosis.
Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) is crucial for cholesteryl ester synthesis and foam cell formation.

Purpose of the Study:

To investigate the role of miR-9 in regulating ACAT1 expression and cellular cholesterol metabolism.
To determine if miR-9 influences foam cell formation, a key event in atherosclerosis.

Main Methods:

Bioinformatic analysis to predict miR-9 binding sites on human ACAT1 mRNA.
Reporter assays (luciferase) to validate miR-9 targeting of ACAT1 3'-untranslated region.
Cell culture experiments using human cell lines (THP-1 derived macrophages) to assess protein levels and foam cell formation.

Main Results:

miR-9 directly targets the 3'-untranslated region of human ACAT1 mRNA.
miR-9 significantly reduces ACAT1 protein levels but not mRNA levels in various human cell lines.
miR-9 functionally inhibits the formation of foam cells from THP-1 derived macrophages.

Conclusions:

miR-9 acts as a post-transcriptional regulator of ACAT1, impacting cellular cholesterol homeostasis.
Reducing ACAT1 protein levels via miR-9 offers a potential therapeutic strategy to decrease foam cell formation and mitigate atherosclerosis progression.