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An Image Guided Transapical Mitral Valve Leaflet Puncture Model of Controlled Volume Overload from Mitral Regurgitation in the Rat
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Plasma proANP and SDMA and microRNAs are associated with chronic mitral regurgitation in a pig model

Susanna Cirera1, Sophia G Moesgaard, Nora E Zois

  • 1Department of Veterinary Clinical and Animal SciencesFaculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark Novo Nordisk A/SMaaloev, Denmark Department of Clinical BiochemistryCopenhagen University Hospital Rigshospitalet, Copenhagen, Denmark Department of Cardiothoracic and Vascular SurgeryAarhus University Hospital, Aarhus, Denmark Department of Clinical BiochemistryAarhus University Hospital, Aarhus, Denmark Department of Veterinary Disease BiologyFaculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark Department of Clinical ChemistryVU University Medical Center, Amsterdam, The Netherlands.

Endocrine Connections
|September 14, 2013
PubMed
Abstract

Insights

Plasma pro-atrial natriuretic peptide (proANP) and symmetric dimethylarginine (SDMA) levels, along with specific microRNAs, correlate with the severity of non-ischemic mitral regurgitation (MR) in a new porcine model.

Area of Science:

  • Cardiovascular Research
  • Translational Medicine
  • Molecular Cardiology

Background:

  • Non-ischemic mitral regurgitation (MR) stems from myxomatous mitral valve disease, causing left ventricular remodeling and dysfunction.
  • Understanding the molecular and plasma marker changes in MR is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate plasma marker regulation and cardiac gene expression in a novel porcine model of non-ischemic MR.
  • To establish a preclinical model for studying the pathophysiology of MR.

Main Methods:

  • Twenty-eight pigs underwent either MR induction or sham surgery.
  • MR severity was classified as mild (mMR) or moderate/severe (sMR) via echocardiography.
  • Plasma markers (proANP, l-arginine, ADMA, SDMA) and cardiac tissue gene/microRNA expression were analyzed.

Main Results:

  • Pigs with sMR showed significantly elevated plasma proANP and SDMA compared to controls and mMR.
  • Differential expression of cardiac genes and microRNAs (miR-21, miR-133a) was observed across heart localizations and experimental groups.
  • Gene expression patterns varied significantly among the mitral valve, papillary muscle, and left ventricular free wall.

Conclusions:

  • Plasma proANP and SDMA levels are sensitive indicators of MR severity in this experimental model.
  • Tissue expression of miR-21 and miR-133a is associated with the degree of chronic MR.
  • The porcine model effectively mimics key aspects of human non-ischemic MR, offering a platform for further research.
Keywords:
SDMAmicroRNAmitral regurgitationpigproANP

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