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Related Experiment Video

Updated: May 7, 2026

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response
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Published on: October 3, 2019

The relevance of 7-day patch test reading.

Eleanor Higgins1, Paul Collins

  • 1From the Department of Dermatology, St Vincent's University Hospital, Dublin, Ireland.

Dermatitis : Contact, Atopic, Occupational, Drug
|September 14, 2013
PubMed
Summary

A day 7 patch test reading helps identify delayed allergic reactions to common allergens like fragrances and preservatives. This extended reading schedule improves diagnostic accuracy for contact dermatitis.

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Last Updated: May 7, 2026

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21:16

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

Published on: July 13, 2009

Area of Science:

  • Dermatology
  • Allergy and Immunology

Background:

  • Standard patch testing involves readings at 48 and 96 hours.
  • Literature suggests delayed allergic reactions can occur beyond 96 hours to various substances.
  • Identifying these late reactions is crucial for accurate diagnosis.

Purpose of the Study:

  • To evaluate the diagnostic benefit of a day 7 (168 hours) patch test reading.
  • To determine the frequency and types of relevant late positive reactions.

Main Methods:

  • 203 consecutive patients underwent patch testing with standard and additional series.
  • Patch tests were applied simultaneously.
  • Readings were performed on days 2, 4, and 7.

Main Results:

  • 12.8% of patients (26/203) showed new positive reactions on day 7.
  • 28 relevant positive reactions were identified across 21 allergens.
  • Commonly identified allergens included fragrances, preservatives, and metals like mercury and cobalt.

Conclusions:

  • A day 7 patch test reading is beneficial for detecting relevant late positive reactions.
  • This extended reading schedule enhances the identification of contact allergies.
  • Supports incorporating day 7 readings into routine patch testing protocols.