An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
Carla Dray Marassi1, Marley Silva, Walter Martin Roland Oelemann
1Laboratório de Bacteriologia Veterinária, Universidade Federal Fluminense , Niterói, RJ , Brasil.
Abstract:
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
More Related Videos
09:54Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
Published on: February 16, 2020
09:34An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
Published on: August 16, 2021
