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Updated: May 7, 2026
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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
16S rRNA region based PCR protocol for identification and subtyping of Parvimonas micra
C Ota-Tsuzuki1, A T P Brunheira, M P A Mayer
1Instituto de Ciências Biomédicas, Universidade de São Paulo , São Paulo, SP , Brasil.
Abstract:
The present study established a PCR protocol in order to identify Parvimonas micra and to evaluate the intra-species diversity by PCR-RFLP of 16S rRNA partial sequence. The data indicated that the protocol was able to identify this species which could be clustered in five genotypes.
