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Updated: May 7, 2026
Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Isolation and identification of Profenofos degrading bacteria
Saadatullah Malghani1, Nivedita Chatterjee, Hu Xue Yu
1Key Laboratory of Biogeology and Environmental Geology, Ministry of Education (BGEG), School of Environmental Studies, China University of Geosciences , 388 Lumo Road, Wuhan, Hubei 430074 , China.
Abstract:
An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96% similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y which showed 99% similarity to the 16S rRNA gene of Burkholderia gladioli. Both strains grew well at pH 5.5-7.2 with a broad temperature profile ranging from 28° to 36 °C. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 ug g(-1); profenofos resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium (FTW) reduction in profenofos concentration was 90% within 96 hours of incubation. A literature survey revealed that no data is available regarding the role of Burkholderia gladioli on pesticide biodegradation as well as on profenofos.
