Related Experiment Video
Updated: May 7, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Occurrence of Staphylococcus aureus in raw milk produced in dairy farms in São Paulo state, Brazil
Helena Fagundes1, Luciana Barchesi, Antonio Nader Filho
1Departamento de Engenharia de Alimentos, Faculdade de Zootecnia e Engenharia de Alimentos , Universidade de São Paulo, Pirassununga, SP , Brasil.
Abstract:
The objective of the present study was to evaluate the occurrence of Staphylococcus aureus in milk produced in 37 farms located in the regions of Ribeirão Preto and São Carlos, state of São Paulo, Brazil. Two-hundred and eight samples of milk from individual cows showing subclinical mastitis, and 37 samples of bulk tank milk were analyzed. S. aureus strains were detected in 18 (7.3%) milk samples: 14 (6.7%) from samples of individual cows, and 4 (10.8%) from bulk tank milk. Two individual milk samples (14.3%) and two bulk milk samples contained enterotoxigenic S. aureus. PFGE analysis revealed the genetic heterogeneity of the strains isolated from raw milk, which presented to 13 S. aureus patterns. Results confirmed the potential transmission of staphylococcal food poisoning to consumers via milk of cows affected by subclinical mastitis, mainly when raw milk is ingested.
Related Concept Videos
Clinical Significance of Antibiotic Resistance
Staphylococcal Skin Infections
Investigation of Disease Outbreaks
Microbes in the Production of Fermented Foods
Mechanism of Antibiotic Resistance in MRSA
Microbes in Beverage Production

