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Updated: May 7, 2026

06:27
Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Eduardo Eustáquio de Souza Figueiredo1, Ricardo Cezar Tavares Carvalho, Flávia Galindo Silvestre
1Universidade Federal do Rio de Janeiro , Rio de Janeiro, RJ , Brasil.
Abstract:
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.