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Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Mycoplasmas hyorhinis in different regions of cuba. diagnosis
Evelyn Lobo1, Carlos Poveda, Rakesh Gupta
1Laboratorio de Diagnóstico de Micoplasmas (MYCOLAB), grupo de Biología Molecular, Dirección de Microbiología , CENSA , Cuba;
Abstract:
M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic region 16S-23S of M. hyorhinis rRNA demonstrated the existing variability among strains of a same species. This study is the first report on M. hyorhinis detection in Cuba.
Insights
Mycoplasma hyorhinis causes pneumonia in pigs, often mistaken for M. hyopneumoniae. This study found M. hyorhinis in 66% of lung samples across Cuba, revealing strain variability.
Area of Science:
- Veterinary Microbiology
- Swine Pathology
Background:
- Mycoplasma hyorhinis is an etiological agent of swine arthritis.
- Certain M. hyorhinis strains can cause pneumonia, mimicking Mycoplasma hyopneumoniae infections.
- Pigs with Enzootic Pneumonia (EP) lesions are often suspected of M. hyopneumoniae.
Purpose of the Study:
- To investigate the prevalence of Mycoplasma hyorhinis in pig lungs with EP lesions in Cuba.
- To identify M. hyorhinis strains and assess their genetic variability.
Main Methods:
- Molecular detection using Polymerase Chain Reaction (PCR) and real-time PCR.
- Analysis of the 16S-23S rRNA intergenic region for strain characterization.
- Examination of 280 lung exudate samples from pigs with typical EP lesions.
Main Results:
- Mycoplasma hyorhinis was detected in 66% of the analyzed samples.
- The presence of M. hyorhinis was confirmed in all studied Cuban provinces.
- Variability among M. hyorhinis strains was observed through 16S-23S rRNA gene analysis.
Conclusions:
- This study provides the first report of Mycoplasma hyorhinis detection in Cuba.
- M. hyorhinis is a significant pathogen in Cuban swine, contributing to pneumonia.
- Genetic diversity exists within M. hyorhinis strains circulating in Cuban swine populations.
