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Published on: June 14, 2024
Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
Leonardo Garcia Monte1, Sérgio Jorge, João Paulo Mesquita Luiz
1Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas , Pelotas, RS , Brasil ; Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas , Pelotas, RS , Brasil.
Abstract:
Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
