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Related Experiment Video

Updated: May 7, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Rhinovirus detection using different PCR-based strategies.

E R M Silva1, A S A Watanabe, E Carraro

  • 1Laboratório de Virologia Clínica, Unidade de Doenças Infecciosas, Departamento de Medicina, Universidade Federal de Sao Paulo , São Paulo, SP , Brasil.

Brazilian Journal of Microbiology : [Publication of the Brazilian Society for Microbiology]
|September 14, 2013
PubMed
Summary
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Detecting human rhinoviruses (HRVs) and enteroviruses (HEVs) requires careful assay selection. This study compared three methods, finding picornavirus detection more sensitive but less specific for identifying HRVs in respiratory infections.

Area of Science:

  • Virology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Human rhinoviruses (HRVs) are a primary cause of the common cold.
  • HRVs are now classified within the Enterovirus genus (HEV) of the Picornaviridae family.
  • HRVs and HEVs share genomic and sequence similarities, complicating detection.

Purpose of the Study:

  • To evaluate and compare the effectiveness of different diagnostic strategies for detecting HRVs.
  • To assess the sensitivity and specificity of various molecular assays for HRV and HEV identification.
  • To determine the optimal method for confirming HRV and HEV infections in patients with acute respiratory illness.

Main Methods:

  • Analysis of 291 adult respiratory infection samples collected between 2001-2003.
Keywords:
DetectionDifferentiationEnterovirusRhinovirus

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Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
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Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Published on: November 10, 2023

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Last Updated: May 7, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
03:53

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV

Published on: November 10, 2023

  • Utilized three distinct molecular assays: RT-PCR/hybridization for picornaviruses, RT-PCR/sequencing for rhinoviruses, and duplex semi-nested-RT-PCR for HRV/HEV differentiation.
  • Concordance analysis between assays and evaluation of discordant samples using a third protocol.

    • Main Results:

    • The picornavirus detection assay showed higher sensitivity but lower specificity compared to rhinovirus-specific assays.
    • RT-PCR/hybridization and RT-PCR/sequencing assays demonstrated 83% concordance.
    • A duplex semi-nested-RT-PCR protocol was found to be less sensitive and ineffective for differentiating HRV from HEV.

    Conclusions:

    • No single assay is ideal for detecting both HRVs and HEVs; assay choice depends on desired specificity and sensitivity.
    • Further investigation into sequencing assays targeting different genes is recommended for accurate confirmation of HRV and HEV infections.
    • The study highlights the challenges in differentiating closely related viruses within the Enterovirus genus using current molecular techniques.