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Related Experiment Video

Updated: May 7, 2026

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Flow-mediated stem cell labeling with superparamagnetic iron oxide nanoparticle clusters.

Nicholas Clay1, Kwanghyun Baek2, Artem Shkumatov3

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, United States.

ACS Applied Materials & Interfaces
|September 17, 2013
PubMed
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Manipulating the cellular mechanical environment using orbital flow significantly increased stem cell uptake of superparamagnetic iron oxide nanoparticle (SPIO) clusters, improving cell tracking and therapies.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Nanotechnology

Background:

  • Superparamagnetic iron oxide nanoparticles (SPIOs) are crucial for cell tracking and imaging.
  • Enhancing SPIO uptake in cells is essential for improving diagnostic and therapeutic applications.

Purpose of the Study:

  • To develop a strategy for increasing SPIO cluster uptake by stem cells.
  • To investigate the impact of the cellular mechanical environment on SPIO ingestion.

Main Methods:

  • Stem cells were cultured under static conditions and exposed to orbital flow.
  • SPIO cluster uptake was quantified in both conditions.
  • Magnetic resonance (MR) contrast was evaluated for SPIO-labeled cells in vitro and in vivo.

Main Results:

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Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
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Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

Related Experiment Videos

Last Updated: May 7, 2026

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles
08:26

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle
05:05

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

Published on: November 4, 2011

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

  • Stem cells exposed to orbital flow ingested nearly double the amount of SPIO clusters compared to static cultures.
  • Improved MR contrast was observed in SPIO-labeled cells within collagen gels and a mouse model.

Conclusions:

  • Manipulating the cellular mechanical environment via orbital flow is an effective strategy to enhance SPIO uptake.
  • This method improves the efficiency of SPIO-based cell tracking and holds promise for cell therapies.