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Mechanical compressive force inhibits adipogenesis of adipose stem cells
1State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
Objective:
The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro.
Materials And Methods:
Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-γ1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR-γ and ph-PPAR-γ at the protein level.
Results:
Our data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-γ1 and APN and protein level of PPAR-γ, in ASCs.
Conclusions:
In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.