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Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: May 7, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Northern blotting.

Shan L He¹, Rachel Green

¹Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Enzymology

|September 17, 2013

Summary

This summary is machine-generated.

This study quantifies the size and abundance of RNA produced by a specific gene. Understanding gene transcription helps in studying gene expression and regulation.

Keywords:

Denaturing agarose gel electrophoresis Hybridization Nylon membrane Radiolabeled RNA probe Transfer RNA

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Published on: October 14, 2010

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Last Updated: May 7, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

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Published on: August 21, 2014

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Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

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Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Gene transcription is the process of creating an RNA copy from a DNA sequence.
Measuring RNA levels is crucial for understanding gene expression and cellular function.

Purpose of the Study:

To accurately determine the size and quantity of RNA transcribed from a specific gene of interest.

Main Methods:

Utilized techniques to isolate and analyze RNA transcripts.
Employed methods for precise measurement of RNA size and amount.

Main Results:

Successfully quantified the size distribution of the target RNA.
Determined the relative abundance of the specific RNA transcript.

Conclusions:

The study provides essential data on the transcriptional output of the gene.
Findings contribute to a deeper understanding of gene regulation and expression patterns.