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Updated: May 7, 2026

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
10:07

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

Published on: November 13, 2014

In vitro transcription from plasmid or PCR-amplified DNA

Julie L Brunelle1, Rachel Green

  • 1Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Enzymology
|September 17, 2013
PubMed

Keywords:
Column purificationIn vitro transcription reactionPCR-amplified DNAPhenol extract DNA TemplateRNA polymerase

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