Polysome profile analysis--yeast

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Martin Pospísek1, Leos Valásek

  • 1Department of Genetics and Microbiology, Faculty of Science, Charles University, Prague, the Czech Republic.

Methods in Enzymology
|September 17, 2013
PubMed

Keywords:
Grow yeast culturesPolysome profile analysisSucrose density gradient centrifugationWestern and Northern blottingYeast whole cell extracts (WCEs)

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Ribosome Profiling

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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...