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Updated: May 7, 2026
Polysome Profiling without Gradient Makers or Fractionation Systems
Published on: June 1, 2021
Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae
1The Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, OH, USA.
Abstract:
The control of mRNA translation is vital for gene expression and it is regulated under various physiological and pathological conditions (Groppo and Richter, 2009). For example, under some physiological conditions, translational initiation is impaired. Therefore, the association of mRNA with ribosomes and/or ribosomal subunits can provide a powerful means to dissect specific aspects of posttranscriptional mRNA regulation. This protocol describes a technique used to determine ribosome occupancy on mRNA.
Insights
This study details a method to measure ribosome occupancy on messenger RNA (mRNA). Understanding mRNA translation control is crucial for analyzing gene expression under various conditions.
Area of Science:
- Molecular Biology
- Gene Expression Regulation
Background:
- mRNA translation control is essential for cellular function.
- Translational initiation can be impaired in certain physiological states.
- Analyzing mRNA-ribosome interactions offers insights into posttranscriptional regulation.
Purpose of the Study:
- To describe a technique for determining ribosome occupancy on mRNA.
- To provide a method for dissecting posttranscriptional mRNA regulation.
Main Methods:
- Protocol for assessing ribosome occupancy on mRNA.
- Technique involves analyzing the association of mRNA with ribosomes.
Main Results:
- The protocol allows for the measurement of ribosome density across mRNA molecules.
- This method can identify specific regulatory mechanisms affecting translation.
Conclusions:
- The described technique is a valuable tool for studying mRNA translation.
- It aids in understanding gene expression control at the posttranscriptional level.
