Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dissecting hydrogen bond energetics to answer the age-old question: "How much do hydrogen bonds contribute to enzymatic catalysis?"

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Smoking drives an epigenetic memory of aberrant hematopoiesis.

medRxiv : the preprint server for health sciences·2026

Same author

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition.

bioRxiv : the preprint server for biology·2026

Same author

Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles.

Cell·2026

Same author

Understanding how enzymes work: the journey to ensemble-function studies.

The FEBS journal·2026

Same author

Proteolytic activation of diverse antiviral defense modules in prokaryotes.

bioRxiv : the preprint server for biology·2025

Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026

Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026

Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026

Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026

Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026

Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 7, 2026

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

RNA radiolabeling.

Rishi Porecha¹, Daniel Herschlag

¹Department of Biochemistry, Stanford University, Stanford, CA, USA.

Methods in Enzymology

|September 17, 2013

Summary

This summary is machine-generated.

Radioactive end-labeling enables sensitive detection of nucleic acids like RNA and DNA. This technique is crucial for studying RNA

Keywords:

Denaturing polyacrylamide gel Dephosphorylation E. coli DNA polymerase klenow fragment Polyacrylamide gel electrophoresis (PAGE) purification RNA extraction RNA radiolabeling

More Related Videos

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Published on: October 27, 2023

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a ¹²⁵I-labeled Azide Prosthetic Group

Published on: October 10, 2016

Related Experiment Videos

Last Updated: May 7, 2026

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Published on: December 20, 2024

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Published on: October 27, 2023

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a ¹²⁵I-labeled Azide Prosthetic Group

Published on: October 10, 2016

Area of Science:

Molecular Biology
Biochemistry
Nucleic Acid Chemistry

Background:

Radioactive end-labeling is a sensitive method for detecting nucleic acids at low concentrations.
This technique is applicable to RNA, DNA, and modified nucleic acids.
RNA molecules often form complex three-dimensional structures in solution.

Purpose of the Study:

To describe protocols for radioactive end-labeling of nucleic acids, particularly RNA.
To highlight the utility of end-labeling for structural analysis of RNA.
To introduce end-labeling as a prerequisite for advanced RNA structural studies.

Main Methods:

Radioactive labeling at the 5'- or 3'-end of nucleic acids.
Application of labeling to RNA, DNA, and modified nucleic acids.
Protocols detailed for radioactive end-labeling procedures.

Main Results:

Successful radioactive end-labeling of nucleic acids, enabling visualization and detection.
Demonstration of end-labeling as a gateway to structural analysis techniques for RNA.
Established protocols provide a foundation for further research in RNA structure and function.

Conclusions:

Radioactive end-labeling is a versatile and essential technique for nucleic acid research.
The method is critical for visualizing fragmented RNA in structural studies.
End-labeling protocols facilitate advanced investigations into RNA's three-dimensional structures.