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Updated: May 7, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
Reverse transcriptase dideoxy sequencing of RNA
1Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Abstract:
During the course of studying the structure and function of RNA molecules and the proteins that they may encode, as well as studies of the expression of a given gene, there often arises a need to learn the nucleotide sequence of an RNA molecule. While the sequence of a particular RNA is ordinarily encompassed within a corresponding DNA sequence, RNA sequencing lends the advantage of giving the transcription initiation and termination sequences, and the sites where modifications from the original DNA sequence occur.
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