Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NLRC5 expression within tumor cells is critical to activate adaptive and innate antitumor immune responses.

Immunotherapy advances·2026
Same author

Mass Spectrometry-based Lactylome and Confirmation of Lactylated Proteins.

Journal of visualized experiments : JoVE·2026
Same author

Non-invasive assessment of inflammatory bowel disease activity using a DIA-derived stool peptidomic signature and machine learning.

Frontiers in molecular biosciences·2026
Same author

Exploring an Intermediate Colorectal Cancer Screening Test Based on Stool Proteomics and Machine Learning for Optimizing the Selection of Patients for Colonoscopy Identified From FIT.

Molecular & cellular proteomics : MCP·2026
Same author

Biopsy-derived colonoid air-liquid interface monolayers reveal persistent mucosal defects in ulcerative colitis patients.

Inflammatory bowel diseases·2026
Same author

Targeting the JAK/STAT Signaling Pathway in Breast Cancer: Leaps and Hurdles.

Biomedicines·2025

Related Experiment Video

Updated: May 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Proteomics methods for subcellular proteome analysis.

Romain Drissi1, Marie-Line Dubois, François-Michel Boisvert

  • 1Department of Anatomy and Cell Biology, Université de Sherbrooke, Québec, Canada.

The FEBS Journal
|September 17, 2013
PubMed
Summary
This summary is machine-generated.

Quantitative proteomics and mass spectrometry (MS) methods enable tracking protein localization within cells. These techniques help understand protein function and dynamics by analyzing organellar composition under various conditions.

Keywords:
MSorganelleproteomicsstable isotope labeling by amino acids in cell culture (SILAC)subcellular compartment

More Related Videos

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

Related Experiment Videos

Last Updated: May 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach
04:25

Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

Published on: August 8, 2025

Area of Science:

  • Cell Biology
  • Proteomics
  • Biochemistry

Background:

  • Determining subcellular protein distribution is complex due to the dynamic and multicompartmental nature of cells.
  • Understanding protein localization is crucial for elucidating cellular functions and dynamics.

Purpose of the Study:

  • To review quantitative mass spectrometry (MS)-based proteomics approaches for analyzing subcellular protein localization.
  • To highlight the application of these methods in characterizing organellar composition and protein dynamics.

Main Methods:

  • Quantitative proteomics workflows combining cellular fractionation with proteomic analysis.
  • Mass spectrometry (MS)-based techniques for identifying protein complements of organelles.
  • Relative protein quantification across different cell culture conditions.

Main Results:

  • These methods reliably identify organellar protein complements.
  • Accurate protein assignment to subcellular locations is achieved.
  • Relative protein quantification provides insights into dynamic changes.

Conclusions:

  • Quantitative MS-based proteomics offers powerful tools for studying protein localization and dynamics.
  • These approaches enhance the understanding of organellar composition and protein functions in various cellular states.